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LOGOS Acridine Orange/Propidium Iodide Stain

CAD $ 293.95
In stock
Overview
SKU
F23001

LOGOS Acridine Orange/Propidium Iodide Stain

Overview
LOGOS Acridine Orange/Propidium Iodide Stain
Overview
Acridine Orange/Propidium Iodide Stain is a cell viability dye that causes viable nucleated cells to fluoresce green and nonviable nucleated cells to fluoresce red. Acridine Orange/Propidium Iodide Stain can be used to assess cell viability with the automated fluorescence cell counters of the LUNA™ family. Acridine orange permeates viable cells and binds to nucleic acids. Binding to dsDNA causes acridine orange to fluoresce green and binding to ssDNA or RNA causes it to fluoresce red. Propidium iodide binds to nucleic acids. Not being able to permeate intact cell membranes, propidium iodide is taken up by nonviable cells and cells with compromised membranes. Once bound to nucleic acids, its fluorescence increases 20-30 fold and causes the cell to fluoresce red. Due to Förster resonance energy transfer (FRET), the propidium iodide signal absorbs the acridine orange signal in nonviable cells, ensuring no double positive results
More Information
For Use With LUNA-FL™ Automated Cell Counter; LUNA-STEM™ Automated Cell Counter; LUNA-II YF™ Automated Yeast Cell Counter; LUNA-FX7™ Automated Cell Counter
Capacity 2 x 0.5 mL
Excitation Wavelength 500⁄526 nm (acridine orange)
Emission Wavelength 533⁄617 nm (propidium iodide)
Product Type Acridine Orange/Propidium Iodide Stain
Appearance Orange-red liquid
Cell Permeability Membrane permeable (acridine orange) / Membrane impermeant (propidium iodide)
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